An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus.
Identifieur interne : 005473 ( Main/Exploration ); précédent : 005472; suivant : 005474An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus.
Auteurs : Elisabetta Traggiai [Suisse] ; Stephan Becker ; Kanta Subbarao ; Larissa Kolesnikova ; Yasushi Uematsu ; Maria Rita Gismondo ; Brian R. Murphy ; Rino Rappuoli ; Antonio LanzavecchiaSource :
- Nature medicine [ 1078-8956 ] ; 2004.
Descripteurs français
- KwdFr :
- Adulte, Amorces ADN, Animaux, Anticorps monoclonaux (isolement et purification), Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Herpèsvirus humain de type 4 (métabolisme), Humains, Immunisation passive (), Lymphocytes B (immunologie), Microscopie immunoélectronique, Modèles animaux de maladie humaine, Mémoire immunologique (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Réaction de polymérisation en chaîne, Souris, Syndrome respiratoire aigu sévère (immunologie), Test ELISA, Tests de neutralisation, Transformation génétique, alpha-Macroglobulines (métabolisme).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Lymphocytes B, Mémoire immunologique, Syndrome respiratoire aigu sévère.
- isolement et purification : Anticorps monoclonaux.
- métabolisme : Glycoprotéines membranaires, Herpèsvirus humain de type 4, Protéines de l'enveloppe virale, alpha-Macroglobulines.
- Adulte, Amorces ADN, Animaux, Cellules Vero, Glycoprotéine de spicule des coronavirus, Humains, Immunisation passive, Microscopie immunoélectronique, Modèles animaux de maladie humaine, Réaction de polymérisation en chaîne, Souris, Test ELISA, Tests de neutralisation, Transformation génétique.
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Monoclonal (isolation & purification), B-Lymphocytes (immunology), Chlorocebus aethiops, DNA Primers, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Herpesvirus 4, Human (metabolism), Humans, Immunization, Passive (methods), Immunologic Memory (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mice, Microscopy, Immunoelectron, Neutralization Tests, Polymerase Chain Reaction, Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Transformation, Genetic, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), alpha-Macroglobulins (metabolism).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , isolation & purification : Antibodies, Monoclonal.
- immunology : B-Lymphocytes, Immunologic Memory, Severe Acute Respiratory Syndrome.
- metabolism : Herpesvirus 4, Human, Membrane Glycoproteins, Viral Envelope Proteins, alpha-Macroglobulins.
- methods : Immunization, Passive.
- Adult, Animals, Chlorocebus aethiops, DNA Primers, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Microscopy, Immunoelectron, Neutralization Tests, Polymerase Chain Reaction, Spike Glycoprotein, Coronavirus, Transformation, Genetic, Vero Cells.
Abstract
Passive serotherapy can confer immediate protection against microbial infection, but methods to rapidly generate human neutralizing monoclonal antibodies are not yet available. We have developed an improved method for Epstein-Barr virus transformation of human B cells. We used this method to analyze the memory repertoire of a patient who recovered from severe acute respiratory syndrome coronavirus (SARS-CoV) infection and to isolate monoclonal antibodies specific for different viral proteins, including 35 antibodies with in vitro neutralizing activity ranging from 10(-8)M to 10(-11)M. One such antibody confers protection in vivo in a mouse model of SARS-CoV infection. These results show that it is possible to interrogate the memory repertoire of immune donors to rapidly and efficiently isolate neutralizing antibodies that have been selected in the course of natural infection.
DOI: 10.1038/nm1080
PubMed: 15247913
Affiliations:
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Le document en format XML
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Murphy</name></author><author><name sortKey="Rappuoli, Rino" sort="Rappuoli, Rino" uniqKey="Rappuoli R" first="Rino" last="Rappuoli">Rino Rappuoli</name></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15247913</idno><idno type="pmid">15247913</idno><idno type="doi">10.1038/nm1080</idno><idno type="wicri:Area/PubMed/Corpus">002C66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C66</idno><idno type="wicri:Area/PubMed/Curation">002C66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002C66</idno><idno type="wicri:Area/PubMed/Checkpoint">002E42</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002E42</idno><idno type="wicri:Area/Ncbi/Merge">000944</idno><idno type="wicri:Area/Ncbi/Curation">000944</idno><idno type="wicri:Area/Ncbi/Checkpoint">000944</idno><idno type="wicri:doubleKey">1078-8956:2004:Traggiai E:an:efficient:method</idno><idno type="wicri:Area/Main/Merge">005915</idno><idno type="wicri:Area/Main/Curation">005473</idno><idno type="wicri:Area/Main/Exploration">005473</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus.</title><author><name sortKey="Traggiai, Elisabetta" sort="Traggiai, Elisabetta" uniqKey="Traggiai E" first="Elisabetta" last="Traggiai">Elisabetta Traggiai</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Research in Biomedicine, Via Vela 6, CH 6500 Belllinzona, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Via Vela 6, CH 6500 Belllinzona</wicri:regionArea><wicri:noRegion>CH 6500 Belllinzona</wicri:noRegion></affiliation></author><author><name sortKey="Becker, Stephan" sort="Becker, Stephan" uniqKey="Becker S" first="Stephan" last="Becker">Stephan Becker</name></author><author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name></author><author><name sortKey="Kolesnikova, Larissa" sort="Kolesnikova, Larissa" uniqKey="Kolesnikova L" first="Larissa" last="Kolesnikova">Larissa Kolesnikova</name></author><author><name sortKey="Uematsu, Yasushi" sort="Uematsu, Yasushi" uniqKey="Uematsu Y" first="Yasushi" last="Uematsu">Yasushi Uematsu</name></author><author><name sortKey="Gismondo, Maria Rita" sort="Gismondo, Maria Rita" uniqKey="Gismondo M" first="Maria Rita" last="Gismondo">Maria Rita Gismondo</name></author><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R" last="Murphy">Brian R. Murphy</name></author><author><name sortKey="Rappuoli, Rino" sort="Rappuoli, Rino" uniqKey="Rappuoli R" first="Rino" last="Rappuoli">Rino Rappuoli</name></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name></author></analytic><series><title level="j">Nature medicine</title><idno type="ISSN">1078-8956</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antibodies, Monoclonal (isolation & purification)</term><term>B-Lymphocytes (immunology)</term><term>Chlorocebus aethiops</term><term>DNA Primers</term><term>Disease Models, Animal</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Herpesvirus 4, Human (metabolism)</term><term>Humans</term><term>Immunization, Passive (methods)</term><term>Immunologic Memory (immunology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Microscopy, Immunoelectron</term><term>Neutralization Tests</term><term>Polymerase Chain Reaction</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Transformation, Genetic</term><term>Vero Cells</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term><term>alpha-Macroglobulins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Amorces ADN</term><term>Animaux</term><term>Anticorps monoclonaux (isolement et purification)</term><term>Cellules Vero</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Herpèsvirus humain de type 4 (métabolisme)</term><term>Humains</term><term>Immunisation passive ()</term><term>Lymphocytes B (immunologie)</term><term>Microscopie immunoélectronique</term><term>Modèles animaux de maladie humaine</term><term>Mémoire immunologique (immunologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Réaction de polymérisation en chaîne</term><term>Souris</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Test ELISA</term><term>Tests de neutralisation</term><term>Transformation génétique</term><term>alpha-Macroglobulines (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes B</term><term>Mémoire immunologique</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Immunologic Memory</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Herpesvirus 4, Human</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>alpha-Macroglobulins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization, Passive</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Herpèsvirus humain de type 4</term><term>Protéines de l'enveloppe virale</term><term>alpha-Macroglobulines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Chlorocebus aethiops</term><term>DNA Primers</term><term>Disease Models, Animal</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Mice</term><term>Microscopy, Immunoelectron</term><term>Neutralization Tests</term><term>Polymerase Chain Reaction</term><term>Spike Glycoprotein, Coronavirus</term><term>Transformation, Genetic</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Amorces ADN</term><term>Animaux</term><term>Cellules Vero</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Immunisation passive</term><term>Microscopie immunoélectronique</term><term>Modèles animaux de maladie humaine</term><term>Réaction de polymérisation en chaîne</term><term>Souris</term><term>Test ELISA</term><term>Tests de neutralisation</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Passive serotherapy can confer immediate protection against microbial infection, but methods to rapidly generate human neutralizing monoclonal antibodies are not yet available. We have developed an improved method for Epstein-Barr virus transformation of human B cells. We used this method to analyze the memory repertoire of a patient who recovered from severe acute respiratory syndrome coronavirus (SARS-CoV) infection and to isolate monoclonal antibodies specific for different viral proteins, including 35 antibodies with in vitro neutralizing activity ranging from 10(-8)M to 10(-11)M. One such antibody confers protection in vivo in a mouse model of SARS-CoV infection. These results show that it is possible to interrogate the memory repertoire of immune donors to rapidly and efficiently isolate neutralizing antibodies that have been selected in the course of natural infection.</div></front></TEI><affiliations><list><country><li>Suisse</li></country></list><tree><noCountry><name sortKey="Becker, Stephan" sort="Becker, Stephan" uniqKey="Becker S" first="Stephan" last="Becker">Stephan Becker</name><name sortKey="Gismondo, Maria Rita" sort="Gismondo, Maria Rita" uniqKey="Gismondo M" first="Maria Rita" last="Gismondo">Maria Rita Gismondo</name><name sortKey="Kolesnikova, Larissa" sort="Kolesnikova, Larissa" uniqKey="Kolesnikova L" first="Larissa" last="Kolesnikova">Larissa Kolesnikova</name><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R" last="Murphy">Brian R. Murphy</name><name sortKey="Rappuoli, Rino" sort="Rappuoli, Rino" uniqKey="Rappuoli R" first="Rino" last="Rappuoli">Rino Rappuoli</name><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name><name sortKey="Uematsu, Yasushi" sort="Uematsu, Yasushi" uniqKey="Uematsu Y" first="Yasushi" last="Uematsu">Yasushi Uematsu</name></noCountry><country name="Suisse"><noRegion><name sortKey="Traggiai, Elisabetta" sort="Traggiai, Elisabetta" uniqKey="Traggiai E" first="Elisabetta" last="Traggiai">Elisabetta Traggiai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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